Synthesis and Characterization of Nickel Oxide Nanostructures for Electrochemical Analysis of Methotrexate

  • Saeed Ahmed Lakho University of Sindh, Jamshoro

Abstract

Metal oxide nanoparticles have found numerous applications in different fields. In this paper, the preparation of nickel oxide nanostructures is given. The nanostructures were synthesized by using the hydrothermal method. The characterization was done with X-ray diffraction (XRD) and scanning electron microscopy (SEM). The newly synthesized nanostructures were utilized as a modifier of the working electrode, i.e., glassy carbon electrode (GCE). The modified GCE exhibited an excellent response towards methotrexate (MTX) anticancer drug. The modified GCE, as compared to bare GCE, showed an increased response towards MTX. In this study, BrittonRobinson buffer (BRB) was selected as a supporting electrolyte having pH 5. By using electrochemical impedance spectroscopy (EIS), the method was found linear in the range of 5-40 µM with a limit of detection and quantification values of 2.4 µM and 7.28 µM, respectively. The method developed by this way was successfully applied for the analysis of MTX from injection formulations. The interference studies were also carried out to check the method's selectivity.

Published
Dec 23, 2021
How to Cite
LAKHO, Saeed Ahmed. Synthesis and Characterization of Nickel Oxide Nanostructures for Electrochemical Analysis of Methotrexate. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 22, n. 2, p. 273-279, dec. 2021. ISSN 2221-5255. Available at: <http://www.pjaec.pk/index.php/pjaec/article/view/801>. Date accessed: 17 jan. 2022. doi: http://dx.doi.org/10.21743/pjaec/2021.12.06.